Modeling the Temperature Field in Frozen Soil under Buildings in the City of Salekhard Taking into Account Temperature Monitoring

Most residential buildings and capital structures in the permafrost zone are constructed on the principle of maintaining the frozen state of the foundation soils. The changing climate and the increasing anthropogenic impact on the environment lead to changes in the boundaries of permafrost. These changes are especially relevant in the areas of piling foundations of residential buildings and other engineering structures located in the northern regions since they can lead to serious accidents caused by the degradation of permafrost and decrease the bearing capacity of the soil in such areas. Therefore, organization of temperature monitoring and forecasting of temperature changes in the soil under the buildings is an actual problem. To solve this problem, we use computer simulation methods of three-dimensional nonstationary thermal fields in the soil in combination with real-time monitoring of the temperature of the soil in thermometric wells. The developed approach is verified by using the temperature monitoring data for a specific residential building in the city of Salekhard. Comparison of the results of numerical calculations with experimental data showed good agreement. Using the developed computer software, nonstationary temperature fields under this building are obtained and, on this basis, the bearing capacities of all piles are calculated and a forecast of their changes in the future is given. To avoid decreasing the bearing capacity of piles it is necessary to prevent the degradation of permafrost and to supply the thermal stabilization of the soil. The proposed approach, based on a combination of the soil temperature monitoring and computer modeling methods, can be used to improve geotechnical monitoring methods. © 2022 by the authors

Automated Monitoring the Temperature under Buildings with Pile Foundations in Salekhard

In the paper, we consider a method of ground temperature monitoring using the thermometric boreholes and computer modeling the residential buildings with the pile foundation in the city of Salekhard; note that it is located in the permafrost zone. Construction of the residential buildings and industrial structures in the permafrost zone and their operation is carried out according to the principle of preserving the frozen state of foundations. For ground temperature monitoring, thermometric boreholes are used. In a given time period, the measured temperatures are transferred to a server for further processing. Information about the temperature is an important factor for the safety of the buildings and it can be used to evaluate the piles bearing capacity. It allows to propose options for the soil thermal stabilization or to eliminate the detected technogenic heat sources. An approach of mathematical modeling to reconstruct the temperature fields in the pile foundation base of a building is discussed taking into account the data of temperature monitoring. 24 boreholes were equipped with more than 400 in-borehole thermal sensors for testing the method under the residential building I. The preliminary modeling is carried out for December and January 2020 for the contact thermal conductivity model with phase transition with the upper part of the geological section typical for Salekhard (the sandy soils). The modeling describes the freezing processes during the months in detail. The thermal monitoring allows to say that the ground in the base of the Residential building I is stable. But there are detected heat transfers near the borehole T1 at the depth of 12–14 m. The combination of monitoring and computer modeling makes it possible to assess the safety of the operation of the residential buildings in cities located in the permafrost zones. © 2021, Russian Geographical Society. All rights reserved

Motor activity dependent and independent functions for Myosin II in cytokinesis contribute to actomyosin ring assembly and contraction in Schizosaccharomyces pombe

Cytokinesis depends on a contractile actomyosin ring in many eukaryotes [1, 2, 3]. Myosin II is a key component of the actomyosin ring, although whether it functions as a motor or as an actin cross-linker to exert its essential role is disputed [1, 4, 5]. In Schizosaccharomyces pombe, the myo2-E1 mutation affects the upper 50 kDa sub-domain of the myosin II heavy chain, and cells carrying this lethal mutation are defective in actomyosin ring assembly at the non-permissive temperature [6, 7]. myo2-E1 also affects actomyosin ring contraction when rings isolated from permissive temperature-grown cells are incubated with ATP [8]. Here we report isolation of a compensatory suppressor mutation in the lower 50 kDa sub-domain (myo2-E1-Sup1) that reverses the inability of myo2-E1 to form colonies at the restrictive temperature. myo2-E1-Sup1 is capable of assembling normal actomyosin rings, although rings isolated from myo2-E1-Sup1 are defective in ATP-dependent contraction in vitro. Furthermore, the product of myo2-E1-Sup1 does not translocate actin filaments in motility assays in vitro. Superimposition of myo2-E1 and myo2-E1-Sup1 on available rigor and blebbistatin-bound myosin II structures suggests that myo2-E1-Sup1 may represent a novel actin translocation-defective allele. Actomyosin ring contraction and viability of myo2-E1-Sup1 cells depend on the late cytokinetic S. pombe myosin II isoform, Myp2p, a non-essential protein that is normally dispensable for actomyosin ring assembly and contraction. Our work reveals that Myo2p may function in two different and essential modes during cytokinesis: a motor activity-independent form that can promote actomyosin ring assembly and a motor activity-dependent form that supports ring contraction

Rapid production of pure recombinant actin isoforms in Pichia pastoris

Actins are major eukaryotic cytoskeletal proteins, which perform many important cell functions, including cell division, cell polarity, wound healing, and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively presently for biochemical studies of actin cytoskeleton from other organisms / cell types. Here we report a rapid and cost-effective method to purify heterologous actins expressed in the yeast Pichia pastoris. Actin is expressed as a fusion with the actin-binding protein thymosin β4 and purified using an affinity tag introduced in the fusion. Following cleavage of thymosin β4 and the affinity tag, highly purified functional full-length actin is liberated. We purify actins from S. cerevisiae, S. pombe, and the β- and γ- isoforms of human actin. We also report a modification of the method that facilitates expression and purification of arginylated actin, a form of actin thought to regulate actin dendritic networks in mammalian cells. The methods we describe can be performed in all laboratories equipped for molecular biology, and should greatly facilitate biochemical and cell biological studies of the actin cytoskeleton

Biochemical applications of surface-enhanced infrared absorption spectroscopy

An overview is presented on the application of surface-enhanced infrared absorption (SEIRA) spectroscopy to biochemical problems. Use of SEIRA results in high surface sensitivity by enhancing the signal of the adsorbed molecule by approximately two orders of magnitude and has the potential to enable new studies, from fundamental aspects to applied sciences. This report surveys studies of DNA and nucleic acid adsorption to gold surfaces, development of immunoassays, electron transfer between metal electrodes and proteins, and protein–protein interactions. Because signal enhancement in SEIRA uses surface properties of the nano-structured metal, the biomaterial must be tethered to the metal without hampering its functionality. Because many biochemical reactions proceed vectorially, their functionality depends on proper orientation of the biomaterial. Thus, surface-modification techniques are addressed that enable control of the proper orientation of proteins on the metal surface. [Figure: see text

Hydrothermal Synthesis of Delafossite-Type Oxides

The syntheses of copper and silver delafossite-type oxides from their constituent binary metal oxides, oxide hydroxides and hydroxides, by low temperature (<210 °C) and low pressure (<20 atm) hydrothermal reactions are described. Particular emphasis is placed on how the acid-base character of a constituent oxide determines its solubility and therefore whether a particular delafossite-type oxide can be synthesized, a strategy utilized by geologists and mineralogists to understand the conditions necessary for the synthesis of various minerals. Thus, the geochemical and corrosion science literature are shown to be useful in understanding the reaction conditions required for the syntheses of delafossite-type oxides and the relationship between reactant metal oxide acid-base character, solubility, aqueous speciation, and product formation. Manipulation of the key parameters, temperature, pressure, pH, and reactant solubility, results in broad families of phase-pure delafossite-type oxides in moderate to high yields for copper, CuBO2 (B) Al, Sc, Cr, Mn, Fe, Co, Ga, and Rh), and silver, AgBO2 (B ) Al, Sc, Fe, Co, Ni, Ga, Rh, In, and Tl)